| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Goat |
| Applications | ELISA,WB-Ti |
| Brand: | Abnova |
| Reference: | PAB6831 |
| Product name: | POU5F1 polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of POU5F1. |
| Gene id: | 5460 |
| Gene name: | POU5F1 |
| Gene alias: | MGC22487|OCT3|OCT4|OTF3|OTF4 |
| Gene description: | POU class 5 homeobox 1 |
| Immunogen: | A synthetic peptide corresponding to human POU5F1. |
| Immunogen sequence/protein sequence: | C-VTTLGSPMHSN |
| Protein accession: | NP_002692.2;NP_9760.34.2 |
| Form: | Liquid |
| Concentration: | 0.5 mg/mL |
| Recommend dilutions: | ELISA (1:16000) Western Blot (1-3 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Specificity: | This antibody is expected to recognize both reported isoforms (NP_002692 and NP_9760.34) |
| Reactivity: | Human |
| Application image: | ![]() |
| Application image note: | POU5F1 polyclonal antibody (Cat # PAB6831) (1 ug/mL) staining of human liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
| Applications: | ELISA,WB-Ti |
| Shipping condition: | Dry Ice |
| Publications: | OCT4 staining in testicular tumors: a sensitive and specific marker for seminoma and embryonal carcinoma.Jones TD, Ulbright TM, Eble JN, Baldridge LA, Cheng L. Am J Surg Pathol. 2004 Jul;28(7):935-40. |