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Data sheet of ADAM12 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Goat |
| Applications | ELISA,IHC,WB-Ti |
More info about ADAM12 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB6697 |
| Product name: | ADAM12 polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of ADAM12. |
| Gene id: | 8038 |
| Gene name: | ADAM12 |
| Gene alias: | MCMP|MCMPMltna|MLTN|MLTNA |
| Gene description: | ADAM metallopeptidase domain 12 |
| Immunogen: | A synthetic peptide corresponding to human ADAM12. |
| Immunogen sequence/protein sequence: | AARPLPVSPARALC |
| Protein accession: | NP_003465;NP_067673 |
| Form: | Liquid |
| Concentration: | 0.5 mg/mL |
| Recommend dilutions: | ELISA (1:64000) Western blot (0.1-0.3 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Specificity: | This antibody is expected to recognize both the longer membrane-bound form of human ADAM12 (NP_003465) and the shorter soluble ADAM12 splice isoform (NP_067673). This antibody does not cross-react with other ADAMS. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | ADAM12 polyclonal antibody (Cat # PAB6697) staining (0.1 ug/mL) of human heart lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
| Applications: | ELISA,IHC,WB-Ti |
| Shipping condition: | Dry Ice |
| Publications: | Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha -actinin-2, is required for myoblast fusion.Galliano MF, Huet C, Frygelius J, Polgren A, Wewer UM, Engvall E. J Biol Chem. 2000 May 5;275(18):13933-9. |