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Data sheet of POLL polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Goat |
| Applications | ELISA,IHC-P |
More info about POLL polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB6543 |
| Product name: | POLL polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of POLL. |
| Gene id: | 27343 |
| Gene name: | POLL |
| Gene alias: | BETA-N|FLJ46002|POL-KAPPA |
| Gene description: | polymerase (DNA directed), lambda |
| Immunogen: | A synthetic peptide corresponding to human POLL. |
| Immunogen sequence/protein sequence: | C-LGLPYREPAERDW |
| Protein accession: | NP_0.37406 |
| Form: | Liquid |
| Concentration: | 0.5 mg/mL |
| Recommend dilutions: | ELISA (1:32000) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Specificity: | This antibody may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 KDa. The overall % of identity is not very high (about 32% in the core) but considering only the that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | POLL polyclonal antibody (Cat # PAB6543, 5 ug/mL) staining of paraffin embedded human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
| Applications: | ELISA,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | DNA polymerase lambda, a novel DNA repair enzyme in human cells.Garcia-Diaz M, Bebenek K, Sabariegos R, Dominguez O, Rodriguez J, Kirchhoff T, Garcia-Palomero E, Picher AJ, Juarez R, Ruiz JF, Kunkel TA, Blanco L. J Biol Chem. 2002 Apr 12;277(15):13184-91. Epub 2002 Jan 30. |