| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Goat |
| Applications | ELISA,WB-Ce,IHC-P |
| Brand: | Abnova |
| Reference: | PAB6301 |
| Product name: | UBE2K polyclonal antibody |
| Product description: | Goat polyclonal antibody raised against synthetic peptide of UBE2K. |
| Gene id: | 3093 |
| Gene name: | UBE2K |
| Gene alias: | DKFZp564C1216|DKFZp686J24237|E2-25K|HIP2|HYPG|LIG |
| Gene description: | ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast) |
| Immunogen: | A synthetic peptide corresponding to C-terminus of human UBE2K. |
| Immunogen sequence/protein sequence: | C-SWDVETATELLLSN |
| Protein accession: | NP_005330.1;NP_001104582.1;NP_001104583.1 |
| Form: | Liquid |
| Concentration: | 0.5 mg/mL |
| Recommend dilutions: | ELISA (1:2000) Western Blot (1-3 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide) |
| Storage instruction: | Store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Goat |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: | ![]() |
| Application image note: | UBE2K polyclonal antibody (Cat # PAB6301) staining (1.5 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
| Applications: | ELISA,WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme.Kalchman MA, Graham RK, Xia G, Koide HB, Hodgson JG, Graham KC, Goldberg YP, Gietz RD, Pickart CM, Hayden MR. J Biol Chem. 1996 Aug 9;271(32):19385-94. |