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Brand | Abnova |
Product type | Primary antibodies |
Host species | Chicken |
Applications | WB,ICC,IHC |
Brand: | Abnova |
Reference: | PAB29058 |
Product name: | HA polyclonal antibody (FITC) |
Product description: | Chicken polyclonal antibody raised against recombinant HA peptide. |
Isotype: | IgY |
Immunogen: | A KLH-conjugated synthetic peptide corresponding to HA tag emulsified in Freund's adjuvant. |
Immunogen sequence/protein sequence: | YPYDVPDYA |
Form: | Liquid |
Recommend dilutions: | Immunocytochemistry Immunohistochemistry(1:250) Western Blot The optimal working dilution should be determined by the end user. |
Storage buffer: | In PBS, pH 7.2 (0.2% BSA, 0.02% sodium azide) |
Storage instruction: | Store at 4°C and avoid from light. For long term storage, dilute the antibody with sterile glycerol and store it at -20°C. Aliquot to avoid repeated freezing and thawing. |
Quality control testing: | Unlabeled antibody was varified with ELISA and western blot. ELISA analysis involved plates coated with peptide conjugated to carrier protein and HRP-labeled goat anti-chicken IgY at 1:10,000 dilution. Under these conditions, the unlabelled chicken anti-HA antibodies (1:10,000 dilution) produced an optical density of >1.0 in 15 minutes. In western blot, the unlabeled antibody at 1:1,000 dilution gave a prominent band with HRP-labeled goat anti-chicken IgY at 1:10,000 dilution. |
Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Conjugate tag: | FITC |
Product type: | Primary antibodies |
Host species: | Chicken |
Applications: | WB,ICC,IHC |
Shipping condition: | Dry Ice |