| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | IHC-P,IF |
| Brand: | Abnova |
| Reference: | PAB20849 |
| Product name: | SPEN polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against recombinant SPEN. |
| Isotype: | IgG |
| Gene id: | 23013 |
| Gene name: | SPEN |
| Gene alias: | KIAA0929|MINT|RP1-134O19.1|SHARP |
| Gene description: | spen homolog, transcriptional regulator (Drosophila) |
| Immunogen: | Recombinant protein corresponding to amino acids of human SPEN. |
| Immunogen sequence/protein sequence: | NRERAYEHSAYGHHERGTGGFDRTRHYDQDYYRDPRERTLQHGLYYASRSRSPNRFDAHDPRYEPRAREQFTLPSVVHRDIYRDDITREVRGRRPERNYQHSRSRSPHSSQSRNQSPQRLASQASRPTRSPSGSGSRSRSSSSDSI |
| Protein accession: | Q96T58 |
| Form: | Liquid |
| Recommend dilutions: | Immunohistochemistry (1:200-1:500) Immunofluorescence (1-4 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: | ![]() |
| Application image note: | Immunofluorescent staining of human cell line U-251 MG with SPEN polyclonal antibody (Cat # PAB20849) at 1-4 ug/mL dilution shows positivity in nuclei but not nucleoli. |
| Applications: | IHC-P,IF |
| Shipping condition: | Dry Ice |
| Publications: | SPEN protein expression and interactions with chromatin in mouse testicular cells.Korfanty J, Stokowy T, Chadalski M, Toma-Jonik A, Vydra N, Widlak P, Wojta? B, Gielniewski B, Widlak W. Reproduction. 2018 Sep;156(3):195-206. doi: 10.1530/REP-18-0046. Epub 2018 Jun 7. |