| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | WB-Ce,IHC-P |
| Brand: | Abnova |
| Reference: | PAB2058 |
| Product name: | EN1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of EN1. |
| Gene id: | 2019 |
| Gene name: | EN1 |
| Gene alias: | - |
| Gene description: | engrailed homeobox 1 |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human EN1. |
| Protein accession: | NP_001417;Q05925 |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (1:1000) Immunohistochemistry (1:10-50) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.09% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: | ![]() |
| Application image note: | Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 polyclonal antibody (Cat # PAB2058) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. |
| Applications: | WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Engrailed-1 negatively regulates beta-catenin transcriptional activity by destabilizing beta-catenin via a glycogen synthase kinase-3beta-independent pathway.Bachar-Dahan L, Goltzmann J, Yaniv A, Gazit A. Mol Biol Cell. 2006 Jun;17(6):2572-80. Epub 2006 Mar 29. |