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Data sheet of PDCD4 (phospho S457) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat,Xenopus |
| Host species | Rabbit |
| Applications | WB,ELISA,IHC-P |
More info about PDCD4 (phospho S457) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB19940 |
| Product name: | PDCD4 (phospho S457) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of PDCD4. |
| Gene id: | 27250 |
| Gene name: | PDCD4 |
| Gene alias: | H731|MGC33046|MGC33047 |
| Gene description: | programmed cell death 4 (neoplastic transformation inhibitor) |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S457 of human PDCD4. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:400000) Western Blot (1:500-1:2000) Immunohistochemistry (21.25-2.5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined. |
| Reactivity: | Human,Mouse,Rat,Xenopus |
| Application image: |  |
| Application image note: | Western blot analysis of recombinant protein with PDCD4 (phospho S457) polyclonal antibody (Cat # PAB19940). Lane 1& 2 each contain 100 ng recombinant PDCD4. Lanes 3& 4 each contain 30 ug of whole cell extract from 293 HEK cells treated with 20 nM TPA and MG132 proteosome inhibitor for 8 hours. The signal can be competed off with peptide phosphorylated at Ser 457 (Lanes 2 & 4) |
| Applications: | WB,ELISA,IHC-P |
| Shipping condition: | Dry Ice |