| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | Dot-Pep,IHC-P |
| Brand: | Abnova |
| Reference: | PAB1965 |
| Product name: | SMAD4 (phospho T277) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of SMAD4. |
| Gene id: | 4089 |
| Gene name: | SMAD4 |
| Gene alias: | DPC4|JIP|MADH4 |
| Gene description: | SMAD family member 4 |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to residues surrounding T277 of human SMAD4. |
| Protein accession: | NP_005350;Q13485 |
| Form: | Liquid |
| Recommend dilutions: | Dot Blot (1:500) Immunohistochemistry (1:10-50) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.09% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: | ![]() |
| Application image note: | Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with SMAD4 (phospho T277) polyclonal antibody (Cat # PAB1965) which was peroxidase-conjugated to the secondary antibody followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
| Applications: | Dot-Pep,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Transforming growth factor-β3 regulates cell junction restructuring via MAPK-mediated mRNA destabilization and Smad-dependent protein degradation of junctional adhesion molecule B (JAM-B).Zhang X, Lui WY Biochim Biophys Acta. 2015 Jun;1849(6):601-11. |