| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | Dot-Pep |
| Brand: | Abnova |
| Reference: | PAB1657 |
| Product name: | BRAF (phospho T400) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of BRAF. |
| Gene id: | 673 |
| Gene name: | BRAF |
| Gene alias: | B-RAF1|BRAF1|FLJ95109|MGC126806|MGC138284|RAFB1 |
| Gene description: | v-raf murine sarcoma viral oncogene homolog B1 |
| Immunogen: | Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T400 of human BRAF. |
| Protein accession: | NP_004324;P15056 |
| Form: | Liquid |
| Recommend dilutions: | Dot Blot (1:500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.09% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: | ![]() |
| Application image note: | Dot blot analysis of BRAF (phospho T400) polyclonal antibody (Cat # PAB1657) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. |
| Applications: | Dot-Pep |
| Shipping condition: | Dry Ice |
| Publications: | Calcium restriction allows cAMP activation of the B-Raf/ERK pathway, switching cells to a cAMP-dependent growth-stimulated phenotype.Yamaguchi T, Wallace DP, Magenheimer BS, Hempson SJ, Grantham JJ, Calvet JP. J Biol Chem. 2004 Sep 24;279(39):40419-30. Epub 2004 Jul 19. |