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Data sheet of Histone H3 (K4me2) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Yeast |
| Host species | Rabbit |
| Applications | ChIP,Dot-Pep,WB-Ce |
More info about Histone H3 (K4me2) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB15453 |
| Product name: | Histone H3 (K4me2) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Histone H3 (K4me2). |
| Immunogen: | Lysine4 dimethylated peptide of Histone H3. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS, pH 7.2 (0.09% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Specificity: | This antibody is expected to react with Histone H3 from yeast to mammals. |
| Reactivity: | Yeast |
| Application image: |  |
| Application image note: | To confrm the specifcity of the antisera a dot blot system was used with amounts of peptides from 10-250 pmoles. Unmodifed, monomethylated, dimethylated and trimethylated peptides surrounding the Lysine 4 site on Histone H3 were spotted in lanes 1-4 respectively. Lanes 5-8 contained peptides starting at residue 6, with the sequence, TARKSTGGKAPRKQLAT, encompassing the residue lysine 9 on Histone H3 that is unmodifed, monomethylated, dimethylated and trimethylated respectively. Lanes 9-12 contained peptides encompassing the residue lysine 27 on Histone H3 that is unmodifed, monomethylated, dimethylated and trimethylated respectively. |
| Applications: | ChIP,Dot-Pep,WB-Ce |
| Shipping condition: | Dry Ice |