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NKX2-5 polyclonal antibody

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Data sheet of NKX2-5 polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityHuman,Mouse,Rat
Host speciesGoat
ApplicationsELISA,WB-Ti

More info about NKX2-5 polyclonal antibody

Brand: Abnova
Reference: PAB14394
Product name: NKX2-5 polyclonal antibody
Product description: Goat polyclonal antibody raised against synthetic peptide of NKX2-5.
Gene id: 1482
Gene name: NKX2-5
Gene alias: CHNG5|CSX|CSX1|NKX2.5|NKX2E|NKX4-1
Gene description: NK2 transcription factor related, locus 5 (Drosophila)
Immunogen: A synthetic peptide corresponding to human NKX2-5.
Immunogen sequence/protein sequence: C-PRAYSDPDPAKDPR
Protein accession: NP_004378.1;NP_001159647.1;NP_001159648.1
Form: Liquid
Concentration: 0.5 mg/mL
Recommend dilutions: ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.
Storage buffer: In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage instruction: Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Product type: Primary antibodies
Host species: Goat
Antigen species / target species: Human
Specificity: Approx 45 KDa band observed in human heart lysates (calculated MW of 34.9 KDa according to NP_004378.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684).
Reactivity: Human,Mouse,Rat
Application image: PAB14394-47-3-1.jpg
Application image note: NKX2-5 polyclonal antibody (Cat # PAB14394) (0.3 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Applications: ELISA,WB-Ti
Shipping condition: Dry Ice
Publications: Perinatal loss of Nkx2-5 results in rapid conduction and contraction defects.Briggs LE, Takeda M, Cuadra AE, Wakimoto H, Marks MH, Walker AJ, Seki T, Oh SP, Lu JT, Sumners C, Raizada MK, Horikoshi N, Weinberg EO, Yasui K, Ikeda Y, Chien KR, Kasahara H.
Circ Res. 2008 Sep 12;103(6):580-90. Epub 2008 Aug 8.

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