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Rel polyclonal antibody

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Data sheet of Rel polyclonal antibody

BrandAbnova
Product typePrimary antibodies
ReactivityFlies,Fruit fly
Host speciesRabbit
ApplicationsELISA,IHC,IF

More info about Rel polyclonal antibody

Brand: Abnova
Reference: PAB14193
Product name: Rel polyclonal antibody
Product description: Rabbit polyclonal antibody raised against synthetic peptide of Rel.
Gene id: 41087
Gene name: Rel
Gene alias: CG11992|ird|ird4|l(3)neo36|unnamed
Gene description: Relish
Immunogen: A synthetic peptide (conjugated with KLH) corresponding to Drosophila Rel.
Form: Lyophilized
Recommend dilutions: ELISA (1:1000-1:5000)
Immunohistochemistry (1:50)
The optimal working dilution should be determined by the end user.
Storage buffer: 1x PBS (pH7.4)
Storage instruction: Store at -20°C. For long term storage store at -80°C.
Aliquot after reconstitution to avoid repeated freezing and thawing.
Product type: Primary antibodies
Host species: Rabbit
Antigen species / target species: Fruit fly
Specificity: This antibody detects specifically target peptide derived from Drosophila Relish by ELISA and Dot Blotting. Also detects cleaved and uncleaved Relish in PFA-fixed Drosphila tissue (see image). Cross reactivity with human, mouse and rat have not been tested.
Reactivity: Flies,Fruit fly
Application image: PAB14193-49-Q6-1.jpg
Application image note: Third instar larva were parenterally challenged with Erwinia carotovora (a natural fly pathogen).
After 2 hours, the larval fat body was dissected, fixed with paraformaldehyde, and immunostained with Rel polyclonal antibody (Cat # PAB14193) (1/50 dilution), followed by secondary incubation with Goat anti-rabbit-Cy5.
Tissues were also stained with SYTO24 DNA stain.
Images captured by confocal microscopy.
Applications: ELISA,IHC,IF
Shipping condition: Dry Ice
Publications: Activation of the Drosophila NF-kappaB factor Relish by rapid endoproteolytic cleavage.Stoven S, Ando I, Kadalayil L, Engstrom Y, Hultmark D.
EMBO Rep. 2000 Oct;1(4):347-52.

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