Brand | Abnova |
Product type | Primary antibodies |
Reactivity | Flies,Fruit fly |
Host species | Rabbit |
Applications | ELISA,IHC,IF |
Brand: | Abnova |
Reference: | PAB14193 |
Product name: | Rel polyclonal antibody |
Product description: | Rabbit polyclonal antibody raised against synthetic peptide of Rel. |
Gene id: | 41087 |
Gene name: | Rel |
Gene alias: | CG11992|ird|ird4|l(3)neo36|unnamed |
Gene description: | Relish |
Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to Drosophila Rel. |
Form: | Lyophilized |
Recommend dilutions: | ELISA (1:1000-1:5000) Immunohistochemistry (1:50) The optimal working dilution should be determined by the end user. |
Storage buffer: | 1x PBS (pH7.4) |
Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot after reconstitution to avoid repeated freezing and thawing. |
Product type: | Primary antibodies |
Host species: | Rabbit |
Antigen species / target species: | Fruit fly |
Specificity: | This antibody detects specifically target peptide derived from Drosophila Relish by ELISA and Dot Blotting. Also detects cleaved and uncleaved Relish in PFA-fixed Drosphila tissue (see image). Cross reactivity with human, mouse and rat have not been tested. |
Reactivity: | Flies,Fruit fly |
Application image: | ![]() |
Application image note: | Third instar larva were parenterally challenged with Erwinia carotovora (a natural fly pathogen). After 2 hours, the larval fat body was dissected, fixed with paraformaldehyde, and immunostained with Rel polyclonal antibody (Cat # PAB14193) (1/50 dilution), followed by secondary incubation with Goat anti-rabbit-Cy5. Tissues were also stained with SYTO24 DNA stain. Images captured by confocal microscopy. |
Applications: | ELISA,IHC,IF |
Shipping condition: | Dry Ice |
Publications: | Activation of the Drosophila NF-kappaB factor Relish by rapid endoproteolytic cleavage.Stoven S, Ando I, Kadalayil L, Engstrom Y, Hultmark D. EMBO Rep. 2000 Oct;1(4):347-52. |