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Data sheet of H3K27me1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | WB,ELISA,ChIP,Dot |
More info about H3K27me1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB14115 |
| Product name: | H3K27me1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of H3K27me1. |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the monomethylated lysine 27 (H3K27me1). |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:100-1:500) Dot Blot (1:20000) Western Blot (1:1000) ChIP (2 ug/ChIP) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS (0.05% sodium azide, 0.05% proclin 300) |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | A Dot Blot analysis was performed to test the cross reactivity of H3K27me1 polyclonal antibody (Cat # PAB14115) with peptides containing other modifications of histone H3 and the unmodified H3K27. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1 : 20,000. Figure 3 shows a high specificity of the antibody for the modification of interest. |
| Applications: | WB,ELISA,ChIP,Dot |
| Shipping condition: | Dry Ice |
| Publications: | Translating the histone code.Jenuwein T, Allis CD. Science. 2001 Aug 10;293(5532):1074-80. |