| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | WB,ELISA,ChIP,Dot |
| Brand: | Abnova |
| Reference: | PAB14059 |
| Product name: | H3R17me2 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of H3R17me2. |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to region of histone H3 containing the asymmetrically dimethylated arginine 17 (H3R17me2 (asym)). |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:1000-1:3000) Dot Blot (1:20000) Western Blot (1:250) ChIP (10-15 ul/ChIP) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In serum (0.05% sodium azide) |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Reactivity: | Human |
| Application image: | ![]() |
| Application image note: | ELISA was performed using a serial dilution of H3R17me2 polyclonal antibody (Cat # PAB14059). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 40,000. |
| Applications: | WB,ELISA,ChIP,Dot |
| Shipping condition: | Dry Ice |
| Publications: | Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system.Zheng C, Hayes JJ. J Biol Chem. 2003 Jun 27;278(26):24217-24. Epub 2003 Apr 15. |