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Data sheet of POU5F1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,IHC,Dot-Pep,WB-Ti |
More info about POU5F1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12773 |
| Product name: | POU5F1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of POU5F1. |
| Gene id: | 5460|18999|294562 |
| Gene name: | POU5F1 |
| Gene alias: | MGC22487|OCT3|OCT4|OTF3|OTF4 |
| Gene description: | POU class 5 homeobox 1 |
| Immunogen: | A synthetic peptide corresponding to internal domain of human, mouse and rat POU5F1. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human,Mouse,Rat |
| Specificity: | This antibody recognizes ~39 KDa of POU5F1. It does not cross react with POU2F1, POU2F2. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | The tissue lysate derived from mouse kidney was immunoprobed by POU5F1 polyclonal antibody (Cat # PAB12773) at 1 : 500. An immunoreactive band is observed around ~34 kDa (1). This band is abolished by pre-incubation with immunizing peptide (2). |
| Applications: | IP,ELISA,IHC,Dot-Pep,WB-Ti |
| Shipping condition: | Dry Ice |
| Publications: | Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1.Zhang Z, Liao B, Xu M, Jin Y. FASEB J. 2007 Oct;21(12):3042-51. Epub 2007 May 11. |