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Data sheet of DYKDDDDK tag polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Tr |
More info about DYKDDDDK tag polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12767 |
| Product name: | DYKDDDDK tag polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptides of DYKDDDDK tag. |
| Immunogen: | A synthetic peptide corresponding to DYKDDDDK tag. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In PBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C or -80°C. Aliquot to avoid repeated freezing and thawing. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Specificity: | This antibody recognizes FLAG tagged fusion proteins. |
| Application image: |  |
| Application image note: | The whole cell lysate from the over-expressed FLAG tagged recombinant protein was resolved onto 10% SDS-PAGE, then transferred onto NC membrane. Followed by an immune-blotting with DYKDDDDK tag polyclonal antibody (Cat # PAB12767) at 1 : 1000. Lane 2 was pre-incubated by immunizing peptide. |
| Applications: | IP,ELISA,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution.Brizzard BL, Chubet RG, Vizard DL. Biotechniques. 1994 Apr;16(4):730-5. |