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Data sheet of STAT5A (phospho Y694) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ti,IHC-P |
More info about STAT5A (phospho Y694) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12756 |
| Product name: | STAT5A (phospho Y694) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of STAT5A. |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Y694 of human, mouse and rat STAT5A. |
| Immunogen sequence/protein sequence: | DGYVK |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (1:100) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human,Mouse,Rat |
| Specificity: | This antibody recognizes STAT5 with a phosphorylated sites of Tyr694. It does not cross-react with non-phosphospecific peptide. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Immunohistochemical staining of human breast cancer tissue stained with STAT5A (phospho Y694) polyclonal antibody (Cat # PAB12756) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
| Applications: | IP,ELISA,WB-Ti,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Cloning of human Stat5B. Reconstitution of interleukin-2-induced Stat5A and Stat5B DNA binding activity in COS-7 cells.Lin JX, Mietz J, Modi WS, John S, Leonard WJ. J Biol Chem. 1996 May 3;271(18):10738-44. |