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Data sheet of PTEN (phospho S385) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,IHC,Dot-Pep,WB-Ti |
More info about PTEN (phospho S385) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12751 |
| Product name: | PTEN (phospho S385) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of PTEN. |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S385 of human, mouse and rat PTEN. |
| Immunogen sequence/protein sequence: | DTSDP |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (1:200) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human,Mouse,Rat |
| Specificity: | This antibody recognizes PTEN with a phosphorylated site at Serine 385. It does not cross-react with non-phosphospecific peptide. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with PTEN (phospho S385) polyclonal antibody (Cat # PAB12751, lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (lane 2). |
| Applications: | IP,ELISA,IHC,Dot-Pep,WB-Ti |
| Shipping condition: | Dry Ice |
| Publications: | Cooperative phosphorylation of the tumor suppressor phosphatase and tensin homologue (PTEN) by casein kinases and glycogen synthase kinase 3beta.Al-Khouri AM, Ma Y, Togo SH, Williams S, Mustelin T. J Biol Chem. 2005 Oct 21;280(42):35195-202. Epub 2005 Aug 17. |