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Data sheet of ROCK2 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | WB,IP,ELISA,IHC |
More info about ROCK2 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12730 |
| Product name: | ROCK2 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of ROCK2. |
| Gene id: | 9475 |
| Gene name: | ROCK2 |
| Gene alias: | KIAA0619 |
| Gene description: | Rho-associated, coiled-coil containing protein kinase 2 |
| Immunogen: | A synthetic peptide corresponding to the epitope -VETFP- without a phosphorylation site surrounding T396 of human ROCK2. |
| Form: | Liquid |
| Recommend dilutions: | ELISA (0.01-0.1 ug/mL) Immunohistochemistry (0.5-2 ug/mL) Immunoprecipitation (2-5 ug/mL) Western Blot (0.1-1 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (antibody stabilizer). |
| Storage instruction: | Store at 4°C for three months. For long term storage store at -20°C to -80°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody recognizes ~165 KDa of human ROCK2 without a phosphorylation site of Thr396. It does not react to phosphorylated protein. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | The cell lysate derived from NIH/3T3 was immunoprecipitated by ROCK2 polyclonal antibody (Cat # PAB12730), then immunoprobed by the same antibody at 1 : 500 (lane 1). Lane 2 is a negative control. |
| Applications: | WB,IP,ELISA,IHC |
| Shipping condition: | Dry Ice |
| Publications: | Functional effects of rho-kinase-dependent phosphorylation of specific sites on cardiac troponin.Vahebi S, Kobayashi T, Warren CM, de Tombe PP, Solaro RJ. Circ Res. 2005 Apr 15;96(7):740-7. Epub 2005 Mar 17. |