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Data sheet of IRS1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | WB,IP,ELISA,IHC,Dot-Pep |
More info about IRS1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12695 |
| Product name: | IRS1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of IRS1. |
| Gene id: | 3667 |
| Gene name: | IRS1 |
| Gene alias: | HIRS-1 |
| Gene description: | insulin receptor substrate 1 |
| Immunogen: | A synthetic peptide corresponding to residues surrounding S312 of human IRS1. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (0.5-2 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody recognizes ~165 KDa of human IRS1 (without phosphorylation at Ser312). |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | The cell lysate derived from insulin stimulated CHO was immunoprecipitated by IRS1 polyclonal antibody (Cat # PAB12695), then immunoprobed by the same antibody at 1 : 500 (Lane 1). Lane 2 is a negative control. |
| Applications: | WB,IP,ELISA,IHC,Dot-Pep |
| Shipping condition: | Dry Ice |
| Publications: | Protein kinase C Theta inhibits insulin signaling by phosphorylating IRS1 at Ser(1101).Li Y, Soos TJ, Li X, Wu J, Degennaro M, Sun X, Littman DR, Birnbaum MJ, Polakiewicz RD. J Biol Chem. 2004 Oct 29;279(44):45304-7. Epub 2004 Sep 10. |