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Data sheet of ESR1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce,IHC-P |
More info about ESR1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12681 |
| Product name: | ESR1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of ESR1. |
| Gene id: | 2099 |
| Gene name: | ESR1 |
| Gene alias: | DKFZp686N23123|ER|ESR|ESRA|Era|NR3A1 |
| Gene description: | estrogen receptor 1 |
| Immunogen: | A synthetic peptide corresponding to residues surrounding S167 of human ESR1. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (1:200) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody recognizes ~67 KDa of human ESR1 without a phosphorylation site of Ser 167. It does not cross react to phosphorylated peptide. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Immunohistochemical staining of human breast cancer tissue stained with ESR1 polyclonal antibody (Cat # PAB12681) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
| Applications: | IP,ELISA,WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Ligand-independent interactions of p160/steroid receptor coactivators and CREB-binding protein (CBP) with estrogen receptor-alpha: regulation by phosphorylation sites in the A/B region depends on other receptor domains.Dutertre M, Smith CL. Mol Endocrinol. 2003 Jul;17(7):1296-314. Epub 2003 Apr 24. |