| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | WB,IP,ELISA,IHC-P |
| Brand: | Abnova |
| Reference: | PAB12680 |
| Product name: | NOS3 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of NOS3. |
| Gene id: | 4846 |
| Gene name: | NOS3 |
| Gene alias: | ECNOS|eNOS |
| Gene description: | nitric oxide synthase 3 (endothelial cell) |
| Immunogen: | A synthetic peptide corresponding to C-terminus of human NOS3. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (1:50) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody only recognizes ~130 KDa of human NOS3. It does not detect human inducible NOS or rat brain NOS. |
| Reactivity: | Human,Mouse,Rat |
| Application image: | ![]() |
| Application image note: | Immunohistochemical staining of human placenta tissue stained with NOS3 polyclonal antibody (Cat # PAB12680) at 1 : 50 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. |
| Applications: | WB,IP,ELISA,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Functional interaction of endothelial nitric oxide synthase with a voltage-dependent anion channel.Sun J, Liao JK. Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13108-13. Epub 2002 Sep 12. |