| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce |
| Brand: | Abnova |
| Reference: | PAB12642 |
| Product name: | PAK4 (phospho S474) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of PAK4. |
| Gene id: | 10298 |
| Gene name: | PAK4 |
| Gene alias: | - |
| Gene description: | p21 protein (Cdc42/Rac)-activated kinase 4 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S474 of human PAK4. |
| Immunogen sequence/protein sequence: | RKSLV |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody recognizes PAK4 at a phosphorylation site of Serine 474 (~68KDa), or serine 602 of PAK5 (~90KDa) or Serine 560 of PAK6 (~80 KDa). It does not cross react to other PAK1, PAK2 or PAK3. |
| Reactivity: | Human,Mouse,Rat |
| Application image: | ![]() |
| Application image note: | The cell lysate derived from HEK 293 cells was immunoprobed by PAK4 (phospho S474) polyclonal antibody (Cat # PAB12642) at 1 : 500. An immunoreactive band is observed around ~ 68kDa (Lane 1). This band is blocked by pre-incubation with immunizing peptide (Lane 2). |
| Applications: | IP,ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Requirement for PAK4 in the anchorage-independent growth of human cancer cell lines.Callow MG, Clairvoyant F, Zhu S, Schryver B, Whyte DB, Bischoff JR, Jallal B, Smeal T. J Biol Chem. 2002 Jan 4;277(1):550-8. Epub 2001 Oct 19. |