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Data sheet of MAP2K1/MAP2K2 (phospho S218/S222) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,Dot-Pep,WB-Ce |
More info about MAP2K1/MAP2K2 (phospho S218/S222) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12635 |
| Product name: | MAP2K1/MAP2K2 (phospho S218/S222) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP2K1/MAP2K2. |
| Gene id: | 5604|5605 |
| Gene name: | MAP2K1 |
| Gene alias: | MAPKK1|MEK1|MKK1|PRKMK1 |
| Gene description: | mitogen-activated protein kinase kinase 1 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S218/S222 of human MAP2K1/MAP2K2. |
| Immunogen sequence/protein sequence: | SMANS |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody recognizes ~44/45 KDa of human MAP2K1/MAP2K2 at the phosphorylation site of Ser 218/222 or MEK2(pS222/226). It does not cross react to non-phospho MEK1/2. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | The whole cell lysates derived from Starved NIH/3T3 were immunoblotted by MAP2K1/MAP2K2 (phospho S218/222) polyclonal antibody (Cat # PAB12635) at 1 : 1000 (lane 1). The lane 2 was a negative control. |
| Applications: | IP,ELISA,Dot-Pep,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Inhibition of the Raf-MEK1/2-ERK1/2 signaling pathway, Bcl-xL down-regulation, and chemosensitization of non-Hodgkin's lymphoma B cells by Rituximab.Jazirehi AR, Vega MI, Chatterjee D, Goodglick L, Bonavida B. Cancer Res. 2004 Oct 1;64(19):7117-26. |