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Data sheet of GSK3B (phospho Y216) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,IHC,WB-Ce |
More info about GSK3B (phospho Y216) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12624 |
| Product name: | GSK3B (phospho Y216) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of GSK3B. |
| Gene id: | 2932 |
| Gene name: | GSK3B |
| Gene alias: | - |
| Gene description: | glycogen synthase kinase 3 beta |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Y216 of human GSK3B. |
| Immunogen sequence/protein sequence: | NVSYI |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody recognizes GSK3B (pY216) with a phosphorylated site at Tyrosine 216. It does not cross-react with non-phosphospecific peptide. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | The NIH/3T3 cell lysates derived from conditioned culture medium (Lane 1) or without treatment (Lane 2) were resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with GSK3B (phospho Y216) polyclonal antibody (Cat # PAB12624) (Lane 1) at 1 : 500. |
| Applications: | IP,ELISA,IHC,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Highly potent and specific GSK-3beta inhibitors that block tau phosphorylation and decrease alpha-synuclein protein expression in a cellular model of Parkinson's disease.Kozikowski AP, Gaisina IN, Petukhov PA, Sridhar J, King LT, Blond SY, Duka T, Rusnak M, Sidhu A. ChemMedChem. 2006 Feb;1(2):256-66. |