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Data sheet of GSK3A (phospho S21) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,IHC,WB-Ce |
More info about GSK3A (phospho S21) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12623 |
| Product name: | GSK3A (phospho S21) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of GSK3A. |
| Gene id: | 2931 |
| Gene name: | GSK3A |
| Gene alias: | DKFZp686D0638 |
| Gene description: | glycogen synthase kinase 3 alpha |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S21 of human GSK3A. |
| Immunogen sequence/protein sequence: | RTSSFA |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody recognizes GSK3A (pS21) with a phosphorylated site at Serine 9. It does not cross-react with non-phosphospecific peptide. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | The NIH/3T3 cell lysate derived from conditioned culture medium was resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with GSK3A (phospho S21) polyclonal antibody (Cat # PAB12623) at 1 : 500 (Lane 1). Lane 2 was pre-incubated with immunizing peptide. |
| Applications: | IP,ELISA,IHC,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Protein kinase C betaII regulates Akt phosphorylation on Ser-473 in a cell type- and stimulus-specific fashion.Kawakami Y, Nishimoto H, Kitaura J, Maeda-Yamamoto M, Kato RM, Littman DR, Leitges M, Rawlings DJ, Kawakami T. J Biol Chem. 2004 Nov 12;279(46):47720-5. Epub 2004 Sep 9. |