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Data sheet of ESR1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | IP,ELISA,IHC,WB-Ce |
More info about ESR1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12620 |
| Product name: | ESR1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of ESR1. |
| Gene id: | 2099 |
| Gene name: | ESR1 |
| Gene alias: | DKFZp686N23123|ER|ESR|ESRA|Era|NR3A1 |
| Gene description: | estrogen receptor 1 |
| Immunogen: | A synthetic peptide corresponding to residues surrounding S305 of human ESR1. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-1 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (1:200) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody recognizes ~67 KDa of ESR1 without phosphorylation site of SESR1 305 (It is confirmed by dot blot). It does not cross react to phosphorylation site of SESR1305 of ESR1. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | The whole cell lysates derived from MCF-7 were immunoblotted by ESR1 polyclonal antibody (Cat # PAB12620) at 1 : 500 (Lane 1). The immunoreactive band is blocked by pre-incubation of immunizing peptide (Lane 2). |
| Applications: | IP,ELISA,IHC,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Ligand-independent interactions of p160/steroid receptor coactivators and CREB-binding protein (CBP) with estrogen receptor-alpha: regulation by phosphorylation sites in the A/B region depends on other receptor domains.Dutertre M, Smith CL. Mol Endocrinol. 2003 Jul;17(7):1296-314. Epub 2003 Apr 24. |