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Data sheet of AKT1 (phospho S473) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | IP,ELISA,IHC,Dot-Pep,WB-Ce |
More info about AKT1 (phospho S473) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12600 |
| Product name: | AKT1 (phospho S473) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1. |
| Gene id: | 207 |
| Gene name: | AKT1 |
| Gene alias: | AKT|MGC99656|PKB|PKB-ALPHA|PRKBA|RAC|RAC-ALPHA |
| Gene description: | v-akt murine thymoma viral oncogene homolog 1 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S473 of human AKT1. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (0.1-0.2 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (1-2 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In TBS, pH 7.2 (BSA, 10% Proclin300) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | The motif corresponding to the major autophosphorylation site of the human AKT1. This antibody does not cross react to the non-phosphorylated AKT1 or with other unrelated phosphorylated serine. Ser473 corresponds to QFSYS in human, rat, mouse, chicken and canis of AKT/PKB proteins. |
| Reactivity: | Human,Mouse,Rat |
| Application image: |  |
| Application image note: | The whole cell lysate derived from PDGF stimulated NIH/3T3 was immunoprobed by AKT1 (phospho S473) polyclonal antibody (Cat # PAB12600) at 1 : 1000. An immunoreactive band was observed around ~ 60 KDa (A). This band is abolished by pre-incubation with immunizing peptide (B). |
| Applications: | IP,ELISA,IHC,Dot-Pep,WB-Ce |
| Shipping condition: | Dry Ice |