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Data sheet of MARCKS polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Rat |
| Host species | Rabbit |
| Applications | ICC,IHC,WB-Ti,IF |
More info about MARCKS polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12564 |
| Product name: | MARCKS polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against full length recombinant MARCKS. |
| Immunogen: | Recombinant protein corresponding to full length MARCKS. |
| Form: | Liquid |
| Recommend dilutions: | Immunocytochemistry (1:5000) Immunofluorescence (1:500-1:1000) Western Blot (1:20000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In antiserum |
| Storage instruction: | Store at 4°C for short term. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Specificity: | The MARCKS protein runs at about 80KDa on SDS-PAGE gels, although the real molecular weight is much lower, at about 31.5KDa. The reason for this is probably because the protein is extremely rich in acidic residues. Such proteins appear to bind less SDS than the average protein, and so migrate in PAGE more slowly than the normal protein. |
| Reactivity: | Rat |
| Application image: |  |
| Application image note: | Western blot analysis of MARCKS in whole rat cortex (Co), cerebellum (Ce), brain stem (BS) and spinal cord (SC) homogenate stained with MARCKS polyclonal antibody (Cat # PAB12564) at dilution of 1 : 10,000. |
| Applications: | ICC,IHC,WB-Ti,IF |
| Shipping condition: | Dry Ice |
| Publications: | Purification of two distinct proteins of approximate Mr 80,000 from human epithelial cells and identification as proper substrates for protein kinase C.Hirai M, Shimizu N. Biochem J. 1990 Sep 15;270(3):583-9. |