| Brand | Abnova | 
| Product type | Primary antibodies | 
| Reactivity | Human | 
| Host species | Mouse | 
| Applications | Flow Cyt | 
| Brand: | Abnova | 
| Reference: | MAB4640 | 
| Product name: | CD53 monoclonal antibody, clone MEM-53 (PE) | 
| Product description: | Mouse monoclonal antibody raised against native CD53. | 
| Clone: | MEM-53 | 
| Isotype: | IgG1 | 
| Gene id: | 963 | 
| Gene name: | CD53 | 
| Gene alias: | MOX44|TSPAN25 | 
| Gene description: | CD53 molecule | 
| Immunogen: | Native purified CD53 from leukocytes of patient suffering from LGL-type leukaemia. | 
| Form: | Liquid | 
| Recommend dilutions: | Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension) The optimal working dilution should be determined by the end user.  | 
| Storage buffer: | In PBS (0.2% BSA, 0.09% sodium azide) | 
| Storage instruction: | Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing.  | 
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. | 
| Conjugate tag: | PE | 
| Product type: | Primary antibodies | 
| Host species: | Mouse | 
| Antigen species / target species: | Human | 
| Specificity: | This antibody reacts with CD53, a 32-40 KDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. This antibody reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 KDa using endoglycosidase F). | 
| Reactivity: | Human | 
| Applications: | Flow Cyt | 
| Shipping condition: | Blue Ice | 
| Publications: | Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY.Szollosi J, Horejsi V, Bene L, Angelisova P, Damjanovich S. J Immunol. 1996 Oct 1;157(7):2939-46.  |