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Data sheet of Hif1a polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse,Primates,Rat |
| Host species | Rabbit |
| Applications | IHC,WB-Ce |
More info about Hif1a polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12138 |
| Product name: | Hif1a polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against partial recombinant Hif1a. |
| Gene id: | 15251 |
| Gene name: | Hif1a |
| Gene alias: | AA959795|HIF-1alpha|HIF1alpha|MOP1 |
| Gene description: | hypoxia inducible factor 1, alpha subunit |
| Immunogen: | Recombinant protein corresponding to amino acids 530-825 of mouse Hif1a. |
| Protein accession: | Q61221 |
| Form: | Liquid |
| Recommend dilutions: | Immunocytochemistry (1:100) Immunofluorescence (1:100) Immunohistochemistry (Frozen sections) (5-10 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (5-10 ug/mL) Western Blot (1:500-1:2000) The optimal working dil |
| Storage buffer: | In PBS (0.05% sodium azide) |
| Storage instruction: | Store at 4°C. Do not freeze. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Specificity: | This antibody is specific to mouse HIF-1 alpha. |
| Reactivity: | Human,Mouse,Primates,Rat |
| Application image: |  |
| Application image note: | Western blot analysis of Hif1a in a hypoxic sample using Hif1a polyclonal antibody (Cat # PAB12138). Lane 1 : CoCl2 treated COS-7 nuclear extract (50 ug, hypoxic). Lane 2 : Untreated COS-7 nuclear extract (50 ug, normoxic). 10 second ECL exposure. |
| Applications: | IHC,WB-Ce |
| Shipping condition: | Blue Ice |
| Publications: | Oxidized Low-Density Lipoprotein Stimulates Macrophage 18F-FDG Uptake via Hypoxia-Inducible Factor-1α Activation Through Nox2-Dependent Reactive Oxygen Species Generation.Lee SJ, Thien Quach CH, Jung KH, Paik JY, Lee JH, Park JW, Lee KH J Nucl Med. 2014 Oct;55(10):1699-705. doi: 10.2967/jnumed.114.139428. Epub 2014 Sep 11. |