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Data sheet of Nefh polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mammals,Mouse,Primates,Rat |
| Host species | Rabbit |
| Applications | WB,IHC-Fr,IHC-P,IF |
More info about Nefh polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12117 |
| Product name: | Nefh polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against partial recombinant Nefh. |
| Gene id: | 24587 |
| Gene name: | Nefh |
| Gene alias: | Nfh |
| Gene description: | neurofilament, heavy polypeptide |
| Immunogen: | Recombinant protein corresponding to C-terminus of rat Nefh. |
| Form: | Liquid |
| Recommend dilutions: | The optimal working dilution should be determined by the end user. |
| Storage buffer: | In antiserum (0.05% sodium azide) |
| Storage instruction: | Store at 4°C for short term. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Rat |
| Specificity: | This antibody specifically recognizes the heavy microfilament subunit (~180-220 KDa). |
| Reactivity: | Human,Mammals,Mouse,Primates,Rat |
| Application image: |  |
| Application image note: | Section of rat spinal cord stained with Nefh polyclonal antibody (Cat # PAB12117) (green) and monoclonal antibody to ubiquitin C-terminal hydrolase 1. The Nefh antibody binds primarily to phosphorylated axonal forms of NF-H, and so stains axons coursing between the large UCHL1 positive neurons. These large cells are a-motorneurons and UCHL1 protein is a major component of the perikarya and dendrites of these cells. |
| Applications: | WB,IHC-Fr,IHC-P,IF |
| Shipping condition: | Dry Ice |
| Publications: | A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H.Harris J, Ayyub C, Shaw G. J Neurosci Res. 1991 Sep;30(1):47-62. |