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Data sheet of PDHA1 (phospho S293) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | WB,ICC,IF |
More info about PDHA1 (phospho S293) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB12079 |
| Product name: | PDHA1 (phospho S293) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of PDHA1. |
| Gene id: | 5160 |
| Gene name: | PDHA1 |
| Gene alias: | PDHA|PDHCE1A|PHE1A |
| Gene description: | pyruvate dehydrogenase (lipoamide) alpha 1 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S293 human PDHA1. |
| Protein accession: | P08559 |
| Form: | Liquid |
| Recommend dilutions: | Immunocytochemistry (1:50-1:200) Immunofluorescence (1:50-1:200) Western Blot (1:1000-1:5000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In Tris-citrate/phosphate buffer, pH 7.0-8.0 (0.09% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody is specific to the phosphorylated Serine 293 form of the PDHE1 alpha protein. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Western blot analysis of PDHA1 in an in vitro autophosphorylation of PDH complex in response to different stimulants with PDHA1 (phospho S293) polyclonal antibody (Cat # PAB12079). Such stimulants are : (-) none, H2O2-hydrogen peroxide, AR-anhydroretinol, Rol-retinol, RA-retinoic acid, PMA, Lipid-PKC lipid activator. Photo courtesy of Dr. Beatrice Hoyos, Memorial Sloan-Kettering Cancer Center. |
| Applications: | WB,ICC,IF |
| Shipping condition: | Dry Ice |
| Publications: | Pyruvate dehydrogenase complex activity controls metabolic and malignant phenotype in cancer cells.McFate T, Mohyeldin A, Lu H, Thakar J, Henriques J, Halim ND, Wu H, Schell MJ, Tsang TM, Teahan O, Zhou S, Califano JA, Jeoung NH, Harris RA, Verma A. J Biol Chem. 2008 Aug 15;283(33):22700-8. Epub 2008 Jun 9. |