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Data sheet of ULP1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Yeast |
| Host species | Rabbit |
| Applications | ELISA,IF,WB-Re |
More info about ULP1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11365 |
| Product name: | ULP1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against recombinant ULP1. |
| Gene id: | 856087 |
| Gene name: | ULP1 |
| Gene alias: | NIB1 |
| Gene description: | Ubl (ubiquitin-like protein)-specific protease that cleaves Smt3p protein conjugates; specifically required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase |
| Immunogen: | Recombinant protein corresponding to Saccharomyces cerevisiae ULP1. |
| Protein accession: | NP_015305;Q02724 |
| Form: | Lyophilized |
| Recommend dilutions: | ELISA (1:4000-1:20000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C on dry atmosphere. After reconstitution with 100 uL of deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Yeast |
| Reactivity: | Yeast |
| Application image: |  |
| Application image note: | Western blot using ULP1 polyclonal antibody (Cat # PAB11365) shows detection of a truncated ULP1 fusion protein (arrowhead). Increasing concentrations of yeast ULP1 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4°C with a 1:1000 dilution of anti-yULP1 antibody in 5% non-fat dry milk inTTBS. Detection occurred using a 1:1,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 3-sec exposure time. |
| Applications: | ELISA,IF,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast.Mossessova E, Lima CD. Mol Cell. 2000 May;5(5):865-76. |