Cart 0 Product Products (empty)
No products
To be determined Shipping
0,00 € Total
Prices are tax excluded
Product successfully added to your shopping cart
Quantity
Total
There are 0 items in your cart. There is 1 item in your cart.
Total products (tax excl.)
Total shipping (tax excl.) To be determined
Total (tax excl.)
Data sheet of RAD9 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Yeast |
| Host species | Rabbit |
| Applications | ELISA,WB-Re |
More info about RAD9 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11362 |
| Product name: | RAD9 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of RAD9. |
| Gene id: | 851803 |
| Gene name: | RAD9 |
| Gene alias: | - |
| Gene description: | DNA damage-dependent checkpoint protein, required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; potential Cdc28p substrate |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to amino acids 1125-1139 residues surrounding S1129 of Saccharomyces cerevisiae RAD9. |
| Protein accession: | Locus:11q13.1-q13.2;OMIM603761;GDB5592334;SwissProtQ99638 |
| Form: | Liquid |
| Recommend dilutions: | Sandwich ELISA (1:5000) Western Blot (1:100-1:500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Yeast |
| Specificity: | This pan reactive polyclonal antibody reacts equally with both phosphorylated and non-phosphorylated yeast Rad9 at S1129. No reactivity is expected against human and mouse homologs. |
| Reactivity: | Yeast |
| Application image: |  |
| Application image note: | Affinity purified antibody to yeast RAD9 (1125-1139 pan reactive) was used at a 1:200 dilution incubated 8 h at room temperature to detect RAD9 by Western blot. Lanes were loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae RAD9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast RAD9, Lane 2 - in vitro phosphorylated wild type yeast RAD9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant RAD9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of RAD9 was by treatment with ATP and Rad53 kinase. |
| Applications: | ELISA,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | Rad53 FHA domain associated with phosphorylated Rad9 in the DNA damage checkpoint.Sun Z, Hsiao J, Fay DS, Stern DF. Science. 1998 Jul 10;281(5374):272-4. |