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Data sheet of RUB1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Yeast |
| Host species | Rabbit |
| Applications | ELISA,WB-Re |
More info about RUB1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11355 |
| Product name: | RUB1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against full length recombinant RUB1. |
| Gene id: | 851717 |
| Gene name: | RUB1 |
| Gene alias: | - |
| Gene description: | Rub1p |
| Immunogen: | Recombinant protein corresponding to full length Saccharomyces cerevisiae RUB1. |
| Form: | Lyophilized |
| Recommend dilutions: | ELISA (1:1000-1:5000) Western Blot (1:500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot after reconstitution to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Yeast |
| Reactivity: | Yeast |
| Application image: |  |
| Application image note: | RUB1 polyclonal antibody (Cat # PAB11355), generated by immunization with full-length, recombinant yeast RUB1, was tested by immunoblot against a yeast cell lysate. A dilution of the antibody between 1:200 and 1:1,000 will show strong reactivity specifically with free RUB1 protein (indicated by arrow) and RUB1 conjugates. In this blot, the antibody was used at a 1:500 dilution and was incubated overnight at 4°C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). |
| Applications: | ELISA,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | The Arabidopsis cullin AtCUL1 is modified by the ubiquitin-related protein RUB1.del Pozo JC, Estelle M. Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15342-7. |