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Data sheet of Sephs2 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Rabbit |
| Applications | WB,IP,ELISA |
More info about Sephs2 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11323 |
| Product name: | Sephs2 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against full length recombinant Sephs2. |
| Gene id: | 20768 |
| Gene name: | Sephs2 |
| Gene alias: | Sps2|Ysg3 |
| Gene description: | selenophosphate synthetase 2 |
| Immunogen: | Recombinant protein corresponding to full length mouse Sephs2. |
| Protein accession: | AAC53024;P97364 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:5000-1:20000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Specificity: | This antibody reacts with mouse SPS2 and shows partial cross-reactivity with SPS1. |
| Reactivity: | Human,Mouse |
| Application image: |  |
| Application image note: | Western blot using Sephs2 polyclonal antibody (Cat # PAB11323) shows detection of Sephs2 in NIH/3T3 cells over-expressing this protein. No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with Sephs2 siRNA. Endogenous Sephs2 can be detected in mouse kidney, liver and testes tissue lysates. Partial cross-reactivity is seen against recombinant SPS1. The primary antibody was used at a 1 : 1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD. |
| Applications: | WB,IP,ELISA |
| Shipping condition: | Dry Ice |
| Publications: | Cloning and functional characterization of human selenophosphate synthetase, an essential component of selenoprotein synthesis.Low SC, Harney JW, Berry MJ. J Biol Chem. 1995 Sep 15;270(37):21659-64. |