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Data sheet of SAE1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chimpanzee,Dog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | WB,ELISA,IF |
More info about SAE1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11270 |
| Product name: | SAE1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against full length recombinant SAE1. |
| Gene id: | 10055 |
| Gene name: | SAE1 |
| Gene alias: | AOS1|FLJ3091|HSPC140|SUA1 |
| Gene description: | SUMO1 activating enzyme subunit 1 |
| Immunogen: | Recombinant GST fusion protein corresponding to full length human SAE1. |
| Protein accession: | Q9UBE0 |
| Form: | Lyophilized |
| Recommend dilutions: | ELISA (1:5000-1:20000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot after reconstitution to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This purified antibody is directed against human SUMO Activating Enzyme E1 protein. |
| Reactivity: | Bovine,Chimpanzee,Dog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Coomassie-stained SDS-PAGE of GST-SAE1 recombinant protein (Panel A) and western blotting (Panel B) of HeLa WC lysate (lane1) and purified recombinant GST-SAE1 (Lane 2) are presented to show specificity of SAE1 polyclonal antibody (Cat # PAB11270). The ~60 KDa band present in ~ 35 ug lysate (green, 800 nm channel) is indicated by the arrowhead. Lane 2 contains 50 ng of purified recombinant GST-SAE1 and lane 3 contains 300 ng of purified GST.Proteins were separated on a 4-20% Tris-Glycine gel by SDS-PAGE andtransferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 2,000. Incubation was overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature. Molecular weight markers are shown for both the coomassie-stained gel and the western blot (Lane M, red, 700 nm channel). IRDye™ 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is at rademark of LI-COR, Inc. SDS-PAGE image courtesy of Proteome Resources, Englewood, CO, http://www.proteomeresources.com. |
| Applications: | WB,ELISA,IF |
| Shipping condition: | Dry Ice |
| Publications: | In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2.Okuma T, Honda R, Ichikawa G, Tsumagari N, Yasuda H. Biochem Biophys Res Commun. 1999 Jan 27;254(3):693-8. |