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Data sheet of SMAD3 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Clawed frog,Frog,Human,Mouse,Pig,Rat,Zebra fish |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IHC-P |
More info about SMAD3 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11263 |
| Product name: | SMAD3 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of SMAD3. |
| Gene id: | 4088 |
| Gene name: | SMAD3 |
| Gene alias: | DKFZp586N0721|DKFZp686J10186|HSPC193|HsT17436|JV15-2|MADH3|MGC60396 |
| Gene description: | SMAD family member 3 |
| Immunogen: | A synthetic peptide corresponding to amino acids 417-425 of human SMAD3. |
| Protein accession: | NP_005893;P84022 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:40000) Western Blot (1:500-1:3000) Immunohistochemistry (1:500-1:3000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated human Smad3. Expect reactivity against Smad1 and Smad5. Negligible reactivity is seen against other Smad family members. |
| Reactivity: | Bovine,Chicken,Clawed frog,Frog,Human,Mouse,Pig,Rat,Zebra fish |
| Application image: |  |
| Application image note: | Western blot using SMAD3 polyclonal antibody (Cat # PAB11263) shows detection of endogenous SMAD3 in both unstimulated and stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/mL TGF-beta (+ lanes) for 1 hour. This reagent recognizes both non-phosphorylated and phosphorylated SMAD3 protein. Personal Communication, Ying Zhang, NIH, CCR, Bethesda, MD. |
| Applications: | ELISA,WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | Receptor-associated Mad homologues synergize as effectors of the TGF-beta response.Zhang Y, Feng X, We R, Derynck R. Nature. 1996 Sep 12;383(6596):168-72. |