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Data sheet of DIABLO polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce,WB-Re |
More info about DIABLO polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11261 |
| Product name: | DIABLO polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against partial recombinant DIABLO. |
| Gene id: | 56616 |
| Gene name: | DIABLO |
| Gene alias: | DIABLO-S|FLJ10537|FLJ25049|SMAC|SMAC3 |
| Gene description: | diablo homolog (Drosophila) |
| Immunogen: | Recombinant His fusion protein corresponding to amino acids 56-239 of human DIABLO. |
| Form: | Liquid |
| Recommend dilutions: | Western Blot (1:1000-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Immunoblot using DIABLO polyclonal antibody (Cat # PAB11261) detects a 26 kDa band when 1 ug of recombinant DIABLO is applied (lane 1). Lane 2 shows DIABLO detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 micro;M etoposide. Recombinant DIABLO migrates slower than the native form because of the His6-tag. The blot was incubated overnight with a 1:1000 dilution of DIABLO polyclonal antibody. |
| Applications: | IP,ELISA,WB-Ce,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition.Du C, Fang M, Li Y, Li L, Wang X. Cell. 2000 Jul 7;102(1):33-42. |