Cart 0 Product Products (empty)
No products
To be determined Shipping
0,00 € Total
Prices are tax excluded
Product successfully added to your shopping cart
Quantity
Total
There are 0 items in your cart. There is 1 item in your cart.
Total products (tax excl.)
Total shipping (tax excl.) To be determined
Total (tax excl.)
Data sheet of SLIT2 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Bovine,Chicken,Clawed frog,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ti |
More info about SLIT2 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB11259 |
| Product name: | SLIT2 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of SLIT2. |
| Gene id: | 9353 |
| Gene name: | SLIT2 |
| Gene alias: | FLJ14420|SLIL3|Slit-2 |
| Gene description: | slit homolog 2 (Drosophila) |
| Immunogen: | A synthetic peptide corresponding to amino acids 484-500 of human SLIT2. |
| Protein accession: | O94813;NP_004778 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:1500-1:6000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Bovine,Chicken,Clawed frog,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Western blot using SLIT2 polyclonal antibody (Cat # PAB11259) shows detection of a band at ~165 KDa (Lane 1) corresponding to SLIT2 present in a chicken spinal cord whole cell lysate (arrowhead). Approximately 30 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 1,350. Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™ 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. |
| Applications: | ELISA,WB-Ti |
| Shipping condition: | Dry Ice |
| Publications: | Frequent epigenetic inactivation of the SLIT2 gene in gliomas.Dallol A, Krex D, Hesson L, Eng C, Maher ER, Latif F. Oncogene. 2003 Jul 17;22(29):4611-6. |