| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human,Mouse |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Tr |
| Brand: | Abnova |
| Reference: | PAB11256 |
| Product name: | RNF25 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of RNF25. |
| Gene id: | 64320 |
| Gene name: | RNF25 |
| Gene alias: | AO7|FLJ13906 |
| Gene description: | ring finger protein 25 |
| Immunogen: | A synthetic peptide corresponding to C-terminus of human RNF25. |
| Protein accession: | NP_071898;Q96BH1 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:40000) Western Blot (1:200-1:2000) Immunoprecipitation (1 ug) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human,Mouse |
| Application image: | ![]() |
| Application image note: | Western blot using RNF25 polyclonal antibody (Cat # PAB11256) shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 3 is a vector only control. The primary antibody diluted to 1 : 1,000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD. |
| Applications: | IP,ELISA,WB-Tr |
| Shipping condition: | Dry Ice |
| Publications: | RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination.Lorick KL, Jensen JP, Fang S, Ong AM, Hatakeyama S, Weissman AM. Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11364-9. |