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Data sheet of MYC-tag polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,IHC,WB-Re,WB-Tr |
More info about MYC-tag polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10345 |
| Product name: | MYC-tag polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptide of MYC-tag. |
| Gene id: | 4609 |
| Gene name: | MYC |
| Gene alias: | bHLHe39|c-Myc |
| Gene description: | v-myc myelocytomatosis viral oncogene homolog (avian) |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to amino acids 410-419 of human MYC-tag. |
| Immunogen sequence/protein sequence: | EQKLISEEDL |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:135000) Western Blot (1:500-1:5000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This affinity purified antibody is directed against human c-Myc and is useful in determining its presence in various assays. This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. This antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | MYC-tag polyclonal antibody (Cat # PAB10345) detects ~60 kDa amino terminal linked MYC-tagged recombinant protein by western blot (arrowhead). Lane 1 contains ~35 ug of lysate from control 293T cells. Lane 2 contains ~35 ug of lysate from 293T cells overexpressing an N-terminal linked recombinant protein. Amino terminal linked MYC recombinant protein was the gift of Brian Conti, University of North Carolina, Chapel Hill, NC. |
| Applications: | ELISA,IHC,WB-Re,WB-Tr |
| Shipping condition: | Dry Ice |