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Data sheet of His tag polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Host species | Rabbit |
| Applications | ELISA,IHC,WB-Re |
More info about His tag polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10339 |
| Product name: | His tag polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic peptides of His tag. |
| Immunogen: | A synthetic peptide (conjugated with KLH) corresponding to His tag. |
| Immunogen sequence/protein sequence: | HHHHHH |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:40000) Western Blot (1:1000-1:3000) Immunohistochemistry (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Specificity: | In western blotting of bacterial extracts, This antibody does not cross-react with endogenous proteins. |
| Application image: |  |
| Application image note: | His tag polyclonal antibody (Cat # PAB10339) detects His-tagged recombinant proteins by western blot. The blot was blocked with 3% BSA in TBST for 45 min at RT. Antibody was incubated with blot at a 1 : 1,000 dilution in TBST with 3% BSA for 1 hour at RT. Detection occurred using HRP Gt-a-Rabbit IgG diluted 1 : 80,000 in blocking buffer for 30 min at RT. Lane 1 was loaded with 12-Epitope Tag Protein Marker Lysate which has the His epitope tag incorporated through a C-terminal linkage (~ 42 KDa). Lane 2 was loaded with His-SUMO-GFP recombinant protein which has the His epitope tag incorporated through an N-terminal linkage (~ 40 KDa). A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. Proteins were transferred to nitrocellulose using standard methods. Molecular weights were estimated by comparison to standards (Lane M). |
| Applications: | ELISA,IHC,WB-Re |
| Shipping condition: | Dry Ice |