Cart 0 Product Products (empty)
No products
To be determined Shipping
0,00 € Total
Prices are tax excluded
Product successfully added to your shopping cart
Quantity
Total
There are 0 items in your cart. There is 1 item in your cart.
Total products (tax excl.)
Total shipping (tax excl.) To be determined
Total (tax excl.)
Data sheet of MTOR (phospho S2448) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IHC-P |
More info about MTOR (phospho S2448) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10335 |
| Product name: | MTOR (phospho S2448) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of MTOR. |
| Gene id: | 2475 |
| Gene name: | MTOR |
| Gene alias: | FRAP|FRAP1|FRAP2|RAFT1|RAPT1 |
| Gene description: | mechanistic target of rapamycin |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S2448 of human MTOR. |
| Protein accession: | P42345 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:15000-1:65000) Western Blot (1:500-1:2000) Immunohistochemistry (5.0 ug/mL) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Reactivity occurs with phosphorylated mTOR from human derived tissues and cells. Reactivity with mouse and rat is suggested based on protein sequence homologies. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Immunoblotting of MTOR (phospho S2448) polyclonal antibody (Cat # PAB10335) is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human MTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/mL) for 20 min prior to harvest. Follow reaction of antibody with a 1 : 2000 dilution of HRP Goat-a-Rabbit IgG for visualization. |
| Applications: | ELISA,WB-Ce,IHC-P |
| Shipping condition: | Dry Ice |
| Publications: | The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif.Nojima H, Tokunaga C, Eguchi S, Oshiro N, Hidayat S, Yoshino K, Hara K, Tanaka N, Avruch J, Yonezawa K. J Biol Chem. 2003 May 2;278(18):15461-4. Epub 2003 Feb 25. |