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Data sheet of Mdm2 (phospho S185) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Mouse |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce |
More info about Mdm2 (phospho S185) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10334 |
| Product name: | Mdm2 (phospho S185) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of Mdm2. |
| Gene id: | 17246 |
| Gene name: | Mdm2 |
| Gene alias: | 1700007J15Rik|AA415488|Mdm-2 |
| Gene description: | transformed mouse 3T3 cell double minute 2 |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding S185 of mouse Mdm2. |
| Protein accession: | P23804 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:3000-1:12000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Mouse |
| Specificity: | Reactivity occurs against Mouse MDM2 pS185 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated mouse MDM2 is minimal by ELISA and western blot. |
| Reactivity: | Mouse |
| Application image: |  |
| Application image note: | Immunoblotting of Mdm2 (phospho S185) polyclonal antibody (Cat # PAB10334) is shown to detect a 102 kDa band (arrow) corresponding to phosphorylated mouse Mdm2 present in a 293T whole cell lysate. Cells were serum-starved for 24 hours prior to harvest. Approximately 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/mL) for 20 min prior to harvest. Follow reaction of antibody with a 1 : 2000 dilution of HRP Goat-a-Rabbit IgG for visualization. |
| Applications: | IP,ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Constitutive and DNA damage inducible activation of pig3 and MDM2 genes by tumor-derived p53 mutant C277Y.Pospisilova S, Siligan C, Ban J, Jug G, Kovar H. Mol Cancer Res. 2004 May;2(5):296-304. |