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Data sheet of AKT1 (phospho S473) polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Chicken,Human,Mouse,Rat |
| Host species | Rabbit |
| Applications | ELISA,WB-Ce,IHC-P,IF |
More info about AKT1 (phospho S473) polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10323 |
| Product name: | AKT1 (phospho S473) polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1. |
| Gene id: | 207 |
| Gene name: | AKT1 |
| Gene alias: | AKT|MGC99656|PKB|PKB-ALPHA|PRKBA|RAC|RAC-ALPHA |
| Gene description: | v-akt murine thymoma viral oncogene homolog 1 |
| Immunogen: | Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S473 of human AKT1. |
| Protein accession: | P31749;AAH00479 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:15000-1:60000) Western Blot (1:200-1:1000) Immunohistochemistry (1:100-1:500) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Synthetic Peptide. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | This antibody is specific to phosphorylated human AKT. Minimal reactivity occurs against non-phosphorylated AKT. |
| Reactivity: | Chicken,Human,Mouse,Rat |
| Application image: |  |
| Application image note: | Immunofluorescence microscopy. Confocal image using AKT1 (phospho S473) polyclonal antibody (Cat # PAB10323) at a 1 : 40 dilution to detect phosphorylated AKT1 (green) in rat cardiomyocytes infected with adenovirus expressing with wild-type AKT1 in conjunction with a texas-red conjugated phalloidin (red) to label filamentous actin. |
| Applications: | ELISA,WB-Ce,IHC-P,IF |
| Shipping condition: | Dry Ice |
| Publications: | PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?Lawlor MA, Alessi DR. J Cell Sci. 2001 Aug;114(Pt 16):2903-10. |