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Data sheet of SUMO1 polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | ELISA,WB-Re |
More info about SUMO1 polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10319 |
| Product name: | SUMO1 polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against a recombinant SUMO1. |
| Gene id: | 7341 |
| Gene name: | SUMO1 |
| Gene alias: | DAP-1|GMP1|OFC10|PIC1|SENP2|SMT3|SMT3C|SMT3H3|SUMO-1|UBL1 |
| Gene description: | SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae) |
| Immunogen: | Recombinant protein corresponding to human SUMO1. |
| Form: | Lyophilized |
| Recommend dilutions: | ELISA (1:4000-1:20000) Western Blot (1:2000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | Lyophilized from 0.02 M KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide). |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Immunoblot of SUMO1 polyclonal antibody (Cat # PAB10319), generated by immunizationwith recombinant human SUMO, was tested by immunoblot against a SUMO-GFP fusion proteinafter cleavage by proteases. Dilution of the antibody between 1 : 1,000 and 1 : 5,000 showed strong reactivity specifically with the SUMO portion of the fusion protein (arrowhead). In this blot the antibody was used at a 1 : 2000 dilution incubated overnight at 4°C in 5% non-fat dry milk in TTBS. Detection occurred using a 1 : 2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. Achemiluminescence system was used for signal detection. Data contributed by M.Malakhov, www.lifesensors.com, personal communication. |
| Applications: | ELISA,WB-Re |
| Shipping condition: | Dry Ice |
| Publications: | SUMO, ubiquitin's mysterious cousin.Muller S, Hoege C, Pyrowolakis G, Jentsch S. Nat Rev Mol Cell Biol. 2001 Mar;2(3):202-10. |