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Data sheet of CDKN1B polyclonal antibody
| Brand | Abnova |
| Product type | Primary antibodies |
| Reactivity | Human |
| Host species | Rabbit |
| Applications | IP,ELISA,WB-Ce |
More info about CDKN1B polyclonal antibody
| Brand: | Abnova |
| Reference: | PAB10300 |
| Product name: | CDKN1B polyclonal antibody |
| Product description: | Rabbit polyclonal antibody raised against full length recombinant CDKN1B. |
| Gene id: | 1027 |
| Gene name: | CDKN1B |
| Gene alias: | CDKN4|KIP1|MEN1B|MEN4|P27KIP1 |
| Gene description: | cyclin-dependent kinase inhibitor 1B (p27, Kip1) |
| Immunogen: | Recombinant protein corresponding to full length human CDKN1B. |
| Protein accession: | P46527;NP_004055 |
| Form: | Liquid |
| Recommend dilutions: | ELISA (1:10000-1:40000) Western Blot (1:1000-1:4000) The optimal working dilution should be determined by the end user. |
| Storage buffer: | In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide) |
| Storage instruction: | Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| Quality control testing: | Antibody Reactive Against Recombinant Protein. |
| Note: | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Product type: | Primary antibodies |
| Host species: | Rabbit |
| Antigen species / target species: | Human |
| Specificity: | Antiserum will specifically react with a p27 protein from human tissue. No reaction was observed against other related tumor suppressor proteins. No reactivity is observed with p27 from mouse. |
| Reactivity: | Human |
| Application image: |  |
| Application image note: | Western blot using CDKN1B polyclonal antibody (Cat # PAB10300) shows detection of CDKN1B protein in MCF-7 whole cell lysate (lanes 1-3) (arrowhead). Separation was achieved using a 4-20% gradient gel. Blocking occurred using 5% BLOTTO. Primary antibody was diluted 1:500 in 1% BLOTTO. The membrane was washed and reacted with a 1 : 10,000 dilution of Dylightâ„¢ 800 conjugated Gt-a-Rabbit IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (Lane M). |
| Applications: | IP,ELISA,WB-Ce |
| Shipping condition: | Dry Ice |
| Publications: | Cloning of a new member of the retinoblastoma gene family (pRb2) which binds to the E1A transforming domain.Mayol X, Grana X, Baldi A, Sang N, Hu Q, Giordano A. Oncogene. 1993 Sep;8(9):2561-6. |